In silico molecular docking validation of procalcitonin-binding aptamer and sepsis diagnosis

Abstract Background Procalcitonin (PCT) is a protein that can be used as biomarker for sepsis detection. Sepsis disease where early diagnosis crucial. Using PCT new alternative overcomes limitations of traditional diagnostic methods. Objective To develop platform detection using novel material called aptamer. Results Aptamers could specifically bind to were selected and various molecular biology analysis methods utilized confirm the binding affinity between aptamers PCT. Additionally, in silico structural was conducted gain more detailed understanding structure aptamer results experiments supporting evidence. Ultimately, an aptamer-based developed its ability detect general serum samples with high sensitivity specificity confirmed. Conclusion Through this study, we able technique We also performed validation studies combination